The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
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Information-enriched characterization of mobile populations. Morphological info from photographs can incorporate to the richness of flow cytometry information. As an example, the figure shows an in any other case conventional apoptosis assay making use of Annexin V and PI, including mobile imaging to characterize cells in Every single populace to expose morphologically distinctive features. These insights couldn't happen to be acquired from multiplex staining by yourself.
As samples are obtained on the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and retailers illustrations or photos of detected events at a level nearly 6,000 pictures for every 2nd, dependant upon the flow rate and impression dimension. For increased versatility, Attune Cytometric Program allows you to alter the graphic capture frequency as needed and select a selected gate for picture seize, if demanded.
Blend acoustic concentrating technology with higher-parameter Investigation for adaptable spectral unmixing and traditional workflows.
Discover new insights into your experimental knowledge by visualizing populations using mixed fluorescence and morphology knowledge. Our software interface wizards allow downsampling, concatenation (merged files) and dimensionality equipment that are built in the software for an intuitive, streamlined details analysis experience.
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In this article, an experienced user has gated singlets confidently. Soon after evaluating the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate is made up of much more than 4% aggregates.
Pair your Attune flow cytometer using an autosampler for increased workflow effectiveness, and insert robotic integration for maximum throughput.
Dependable image high-quality even at superior throughput Acoustic concentrating and a high-pace digicam Mix to graphic these T cells constantly at small or superior flow fees. Conveniently change target and digicam settings to fulfill experimental prerequisites.
Early signs of this contamination experienced originally been dismissed as debris. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological characteristics to find attention-grabbing subpopulations that will not be apparent from flow cytometry knowledge alone.
Customers can see event images in actual-time to confirm sample top quality, optimize protocols, and record the morphology of each and every mobile populace identified for analysis. Imaging seize also Gains from acoustic focusing, which helps to posture the cells so that a pointy, centered graphic is received.
Seem waves permit precise mobile alignment, causing Excellent facts excellent and ~10X speedier sample flow prices.
Application note: Automatic impression Evaluation reduces https://edwinoalxi.wikihearsay.com/3284519/flow_cytometer_a_revolutionary_tool_for_cell_analysis person-to-person variability in flow cytometry gating approaches
Leverage the strength of Innovative technologies and innovation to speed up your discoveries. Believe in within the Attune Flow Cytometer family to deliver the trustworthiness, speed, and accuracy that established your get the job done apart.
Versatile payment prepare from Agilent Financial Answers enable you to receive the significant instrumentation you need for speedy undertaking, or to improve your lab.
At the guts of each Attune Flow Cytometer lies our innovative acoustic focusing technological know-how, which utilizes audio waves to meticulously align cells within the flow cell. This ground breaking strategy surpasses conventional hydrodynamic strategies, causing faster processing speeds, Extraordinary details quality, and greater sample throughput.
The Attune Xenith cytometer is really a higher-parameter spectral analyzer, delivering equally spectral unmixing and conventional flow abilities for thorough and multipurpose mobile Evaluation.
Agilent Price Promise ensures you no less than 10 years of instrument use from the day you purchase. Usually, we will credit history you Along with the residual price of the system towards an upgraded model.
What gave the impression to be a homogeneous populace is in fact more assorted—an Perception that needs to be regarded when interpreting benefits.